Prevalence of Multi-Drug Resistant Bacteria Associated With Various Yoghurt Products Commonly Sold in Minna, North Central, Nigeria

Mary Okoye Uche; Anthony Okafor  Kelechi

doi:10.4314/just.v41i3.1636

Ms. Uche Mary Okoye Microbiology Department of Federal University of Technology Minna, Nigeria
Mr. Kelechi Anthony Okafor Microbiology Department of Federal University of Technology Minna

DOI: https://doi.org/10.4314/just.v41i3.1636

Keywords: Yoghurt, Multi-drug, Resistant Bacteria, Antibiotic Susceptibility Test

Abstract

The predominance of Multidrug resistant (MDR) bacteria among dairy products especially yoghurt is fast becoming a major concern in most communities. Five (5) industrially prepared yoghurts were aseptically collected and transported to the Microbiology Laboratory of Federal University of Technology, Minna. Samples were serially diluted and inoculated on various media through the pour plate method. The bacterial isolates were identified through their Gram reaction and other biochemical tests. The antibiotic susceptibility tests were carried out using the disc diffusion method on Muller Hinton agar. The result revealed that, sample C (1.7x103) had the highestmicrobial count while Sample D (8x102) had the lowest microbial count respectively. Various bacterial pathogens were isolated and identified with Klebsiella sp, Staphylococcus sp and Escherichia coli having the highest frequency of occurrence (20%), followed by Pseudomonas sp and Lactobaacillus sp which had their frequency of occurrence to be 13.3% while the least frequency of occurrence (6.7%) was observed in Salmonella sp and Streptococcus sp. The antibiotic susceptibility tests revealed that all bacterial isolates were multidrug resistant and as such are a great threat to the general public especially the consumers of yoghurt drinks. Hence, there is a need for adequate and continuous surveillance by food regulatory bodies in Nigeria, to curtailthe infections associated with Multidrug resistant bacteria.

Author Biographies

Ms. Uche Mary Okoye, Microbiology Department of Federal University of Technology Minna, Nigeria

Uche Mary Okoye is a Lecturer in Microbiology Department of Federal University of Technology Minna, Nigeria. She became a staff of the Department in April 2018 after obtaining her B.Tech and M.Tech degrees. She has recently defended her PhD thesis in Medical Microbiology in the same University. Uche has published a number of peer-reviewed journal articles in reputable national and international journals. Her current research interest includes infectious disease and public health, host-pathogen interactions and antibiotics resistant bacteria associated with high morbidities and mortalties in developing countries. She is a member of Nigerian Society of Microbiology (NSM), American Society of Microbiology (ASM) and Society for Experimental Biology of Nigeria (NISEB). She is happily married to Engr. Jude Okoye and is blessed with children.

Mr. Kelechi Anthony Okafor , Microbiology Department of Federal University of Technology Minna

Kelechi Anthony Okafor is a graduate of Microbiology Department of Federal University of Technology Minna, Nigeria. He graduated in 2021 with a B.Tech degree in General Microbiology. He is blessed with his parents and siblings.